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human genome wide crispr cas9 knockout pooled library geckov2  (Genechem)

 
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    Genechem human genome wide crispr cas9 knockout pooled library geckov2
    Human Genome Wide Crispr Cas9 Knockout Pooled Library Geckov2, supplied by Genechem, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/geckov2+crispr+knockout+pooled+library/cas9+crispr+lentiviral+system/pm41612594-429-9-19
    Average 86 stars, based on 1 article reviews
    human genome wide crispr cas9 knockout pooled library geckov2 - by Bioz Stars, 2026-09
    86/100 stars

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    Related Articles

    CRISPR:

    Article Title: A Genome-Scale CRISPR Knock-Out Screen Identifies MicroRNA-5197-5p as a Promising Radiosensitive Biomarker in Colorectal Cancer
    Article Snippet: .. The human GeCKOv2 CRISPR knockout pooled library was synthesized and purchased from Genechem Co, Ltd., Shanghai, China. .. Prior to the screening, the sgRNA library was sequenced by Genechem Co, Ltd., Shanghai, China to verify that all sgRNAs were presented.

    Knock-Out:

    Article Title: A Genome-Scale CRISPR Knock-Out Screen Identifies MicroRNA-5197-5p as a Promising Radiosensitive Biomarker in Colorectal Cancer
    Article Snippet: .. The human GeCKOv2 CRISPR knockout pooled library was synthesized and purchased from Genechem Co, Ltd., Shanghai, China. .. Prior to the screening, the sgRNA library was sequenced by Genechem Co, Ltd., Shanghai, China to verify that all sgRNAs were presented.

    Synthesized:

    Article Title: A Genome-Scale CRISPR Knock-Out Screen Identifies MicroRNA-5197-5p as a Promising Radiosensitive Biomarker in Colorectal Cancer
    Article Snippet: .. The human GeCKOv2 CRISPR knockout pooled library was synthesized and purchased from Genechem Co, Ltd., Shanghai, China. .. Prior to the screening, the sgRNA library was sequenced by Genechem Co, Ltd., Shanghai, China to verify that all sgRNAs were presented.



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    Image Search Results


    In vivo genome-wide CRISPR screening in PDAC. a Schematic representation of the loss-of-function genome-wide screen using the human lentiviral CRISPR/Cas9 library (GeCKOv2) library A in pancreatic ductal adenocarcinoma cell line (HPAF-II). b Average mass of extracted tumor from NSG mice subcutaneous transplanted with 30 million cells and grown for 28 days. Mean of three independent infection replicate experiments ( n = 5, 1 mouse per biological replicate was randomly selected for deep sequencing). Data are represented as mean ± SEM. c Normalized read count distribution from sequenced amplicons. d The unmapped percentage of sgRNAs in the library in cells before transplantation ( n = 3), and tumor samples ( n = 3) on day 28. e Statistical dispersion graphic (Gini index) of the sgRNA distribution within samples from cells and tumor replicates. f Cumulative distribution function (CDF) of library sgRNAs in the cell representation and tumor sample replicates. Shifts in tumor samples reflect altered read counts in subset of sgRNAs. g Pearson correlation of the sgRNA reads between all samples from in vitro and in vivo

    Journal: Molecular Cancer

    Article Title: Identification of HSPE1 as a new actionable cancer vulnerability leads to an innovative and effective combination therapy for pancreatic ductal adenocarcinoma

    doi: 10.1186/s12943-026-02587-9

    Figure Lengend Snippet: In vivo genome-wide CRISPR screening in PDAC. a Schematic representation of the loss-of-function genome-wide screen using the human lentiviral CRISPR/Cas9 library (GeCKOv2) library A in pancreatic ductal adenocarcinoma cell line (HPAF-II). b Average mass of extracted tumor from NSG mice subcutaneous transplanted with 30 million cells and grown for 28 days. Mean of three independent infection replicate experiments ( n = 5, 1 mouse per biological replicate was randomly selected for deep sequencing). Data are represented as mean ± SEM. c Normalized read count distribution from sequenced amplicons. d The unmapped percentage of sgRNAs in the library in cells before transplantation ( n = 3), and tumor samples ( n = 3) on day 28. e Statistical dispersion graphic (Gini index) of the sgRNA distribution within samples from cells and tumor replicates. f Cumulative distribution function (CDF) of library sgRNAs in the cell representation and tumor sample replicates. Shifts in tumor samples reflect altered read counts in subset of sgRNAs. g Pearson correlation of the sgRNA reads between all samples from in vitro and in vivo

    Article Snippet: Human genome-wide CRISPR/cas9 knockout pooled library GeCKOv2 was a gift from Feng Zhang (Addgene#1,000,000,048).

    Techniques: In Vivo, Genome Wide, CRISPR, Infection, Sequencing, Transplantation Assay, Dispersion, In Vitro